Coding

Part:BBa_K2959003:Design

Designed by: Gabriela Carolina Gajón Robles   Group: iGEM19_Tec-Chihuahua   (2019-09-15)


Pinus sylvestris Defensin 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of PsDef1 is preceded by a 6x His-Tag to facilitate its purification by immobilized metal affinity chromatography.


Source

Amino acid sequence retrieved from Kovalyova et al. (2007)

References

Kovalyova, V. A., Gout, I. T., Kyamova, R. G., Filonenko, V. V., & Gout, R. T. (2007). Cloning and analysis of defensin 1 cDNA from Scots pine. Biopolymers and Cell,23(5), 398-404. doi: http://dx.doi.org/10.7124/bc.000779